Exhibit 164 · Laboratory study
Evidence against a role for SV40 infection in human mesotheliomas and high risk of false-positive PCR results owing to presence of SV40 sequences in common laboratory plasmids
The risk of false-positive results due to contamination by common laboratory plasmids containing SV40 sequences has been underestimated.
In 71 mesotheliomas, two PCR primer sets gave positive results at rates similar to earlier positive studies (56–62 percent), but those primers lay in a region of the SV40 T-antigen gene present in common laboratory plasmids. The SV40 sequences found carried a deletion present only in plasmids. All 71 tumors were negative for T-antigen transcripts and T-antigen protein. The authors concluded that the risk of false-positive results from plasmid contamination had been underestimated.
- Source
- Fernando López-Ríos, Peter B. Illei, Valerie Rusch, Marc Ladanyi; The Lancet 364:1157–1166
- Date
- 25 September 2004
- Quoted from
- Abstract, Interpretation
- Grade
- Peer-reviewed
- Document
- https://doi.org/10.1016/S0140-6736(04)17102-X
- Accessed
- 2026-11-09
- Cited by
- Findings citing this exhibit